Plasmid
pEBS

Part:BBa_K1019008:Design

Designed by: Boris Dyakov   Group: iGEM13_Toronto   (2013-09-16)


pEBS Expression Plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1529
    Illegal suffix found in sequence at 1570
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1529
    Illegal SpeI site found at 1571
    Illegal PstI site found at 1585
    Illegal NotI site found at 1535
    Illegal NotI site found at 1578
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1529
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1529
    Illegal suffix found in sequence at 1571
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1529
    Illegal XbaI site found at 1544
    Illegal SpeI site found at 1571
    Illegal PstI site found at 1585
    Illegal NgoMIV site found at 3551
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1913
    Illegal BsaI site found at 2981
    Illegal SapI.rc site found at 4558


Design Notes

The plasmid was created by excising the maltose binding domain of the original pMAL plasmid and then using In-Fusion cloning to insert a consensus region containing the features needed.


Source

Derived from the pMAL plasmid.

References